{"product_id":"proteintech-55054-1-ap","title":"Proteintech, 55054-1-AP, USP9X Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe USP9X (55054-1-AP) by Proteintech is a Polyclonal antibody targeting USP9X in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n55054-1-AP targets USP9X in WB, IHC, IF\/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  K-562 cells,  mouse brain tissue,  rat brain tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human pancreas cancer tissue, human placenta tissue,  mouse small intestine tissue,  rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nUSP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta\/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5\/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ubiquitin specific peptidase 9, X-linked\nCalculated Molecular Weight: 292 kDa\nObserved Molecular Weight: 260-290 kDa\nGenBank Accession Number: NM_001039590\nGene Symbol: USP9X\nGene ID (NCBI): 8239\nRRID: AB_10792932\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q93008\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868847460521,"sku":"55054-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-55054-1-ap","provider":"Iright","version":"1.0","type":"link"}