{"product_id":"proteintech-55120-1-ap","title":"Proteintech, 55120-1-AP, PUMA Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PUMA (55120-1-AP) by Proteintech is a Polyclonal antibody targeting PUMA in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n55120-1-AP targets PUMA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SH-SY5Y cells, SK-N-SH cells,  mouse brain tissue,  rat brain tissue\nPositive IP detected in: mouse heart tissue\nPositive IHC detected in: human testis tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPUMA, also named as JFY-1 and BBC3, belongs to the Bcl-2 family. It is a critical mediator of p53-dependent and -independent apoptosis induced by a wide variety of stimuli. It serves as a proximal signaling molecule whose expression is regulated by transcription factors in response to these stimuli. PUMA transduces death signals primarily to the mitochondria, where it acts indirectly on the Bcl-2 family members Bax and\/or Bak by relieving the inhibition imposed by antiapoptotic members. It directly binds and antagonizes all known antiapoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. PUMA ablation or inhibition leads to apoptosis deficiency underlying increased risks for cancer development and therapeutic resistance. It is a general sensor of cell death stimuli and a promising drug target for cancer therapy and tissue damage. It is essential mediator of p53-dependent and p53-independent apoptosis (PMID: 19641508). Catalog #55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, zebrafish\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: BCL2 binding component 3\nCalculated Molecular Weight: 21 kDa\nObserved Molecular Weight: 18-21 kDa\nGenBank Accession Number: NM_014417\nGene Symbol: PUMA\nGene ID (NCBI): 27113\nRRID: AB_10859944\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BXH1\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868834517161,"sku":"55120-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-55120-1-ap","provider":"Iright","version":"1.0","type":"link"}