{"product_id":"proteintech-55377-1-ap","title":"Proteintech, 55377-1-AP, SETD2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SETD2 (55377-1-AP) by Proteintech is a Polyclonal antibody targeting SETD2 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n55377-1-AP targets SETD2 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, U2OS cells,  K-562 cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human colon cancer tissue, human oesophagus cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumor suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: SET domain containing 2\nCalculated Molecular Weight: 288 kDa\nObserved Molecular Weight: 287 kDa\nGenBank Accession Number: NM_014159\nGene Symbol: SETD2\nGene ID (NCBI): 29072\nRRID: AB_2881318\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BYW2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869384396969,"sku":"55377-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-55377-1-ap","provider":"Iright","version":"1.0","type":"link"}