{"product_id":"proteintech-60162-1-ig","title":"Proteintech, 60162-1-Ig, LCK Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe LCK (60162-1-Ig) by Proteintech is a Monoclonal antibody targeting LCK in WB, IHC, IF\/ICC, IF-P, FC (Intra), ELISA applications with reactivity to human, rat, pig samples\n60162-1-Ig targets LCK in WB, IHC, IF\/ICC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: NK-92 cells, Jurkat cells,  pig thymus tissue,  Ramos cells,  rat thymus tissue,  MOLT-4 cells,  MJ cells\nPositive IHC detected in: human tonsillitis tissue, human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human tonsillitis tissue\nPositive IF\/ICC detected in: HepG2 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and  a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat, pig\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag7694 Product name: Recombinant human LCK protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 236-539 aa of BC013200 Sequence: DEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENDTLLDSQLEEKGLGASPWGNLGQQLLLLPTGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP Predict reactive species\nFull Name: lymphocyte-specific protein tyrosine kinase\nCalculated Molecular Weight: 539 aa, 56 kDa\nObserved Molecular Weight: 50-60 kDa\nGenBank Accession Number: BC013200\nGene Symbol: LCK\nGene ID (NCBI): 3932\nRRID: AB_10663101\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P06239\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888021360809,"sku":"60162-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-60162-1-ig","provider":"Iright","version":"1.0","type":"link"}