{"product_id":"proteintech-60187-1-ig","title":"Proteintech, 60187-1-Ig, Cytokeratin 19 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Cytokeratin 19 (60187-1-Ig) by Proteintech is a Monoclonal antibody targeting Cytokeratin 19 in WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig samples\n60187-1-Ig targets Cytokeratin 19 in WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, HaCaT cells,  PC-3 cells,  HeLa cells,  T-47D cells,  pig colon tissue,  rat colon tissue,  mouse colon tissue\nPositive IHC-Autostainer detected in: human colon tissue\nPositive IHC detected in: human colon tissue, human stomach cancer tissue,  human lung cancer tissue,  human thyroid cancer tissue,  human breast cancer tissue,  human oesophagus cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human breast cancer tissue\nPositive IF-Fro detected in: mouse breast cancer\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:20000-1:100000\nImmunohistochemistry (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:12500-1:50000\nImmunohistochemistry (IHC): IHC : 1:1000-1:51200\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\nImmunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag7407 Product name: Recombinant human KRT19 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-400 aa of BC002539 Sequence: MTSYSYRQSSATSSFGGLGGGSVRFGPGVAFRAPSIHGGSGGRGVSVSSARFVSSSSSGGYGGGYGGVLTASDGLLAGNEKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL Predict reactive species\nFull Name: keratin 19\nCalculated Molecular Weight: 44 kDa\nObserved Molecular Weight: 48 kDa\nGenBank Accession Number: BC002539\nGene Symbol: Cytokeratin 19\nGene ID (NCBI): 3880\nRRID: AB_10859834\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P08727\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887989149865,"sku":"60187-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-60187-1-ig","provider":"Iright","version":"1.0","type":"link"}