{"product_id":"proteintech-60479-1-ig","title":"Proteintech, 60479-1-Ig, Phospho-STAT3 (Ser727) Monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-STAT3 (Ser727) (60479-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-STAT3 (Ser727) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n60479-1-Ig targets Phospho-STAT3 (Ser727) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, A549 cells,  UV treated HSC-T6 cells,  MCF-7 cells,  NIH\/3T3 cells,  EGF treated A549 cells,  UV treated HeLa cells,  TNF alpha treated MCF-7 cells\nPositive IF\/ICC detected in: UV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, HeLa cells\nPositive FC (Intra) detected in: UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSignal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, rat\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: signal transducer and activator of transcription 3 (acute-phase response factor)\nCalculated Molecular Weight: 770 aa, 88 kDa\nObserved Molecular Weight: 85-90 kDa\nGenBank Accession Number: BC000627\nGene Symbol: STAT3\nGene ID (NCBI): 6774\nRRID: AB_3670262\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P40763\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888031846569,"sku":"60479-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-60479-1-ig","provider":"Iright","version":"1.0","type":"link"}