{"product_id":"proteintech-66003-1-ig","title":"Proteintech, 66003-1-Ig, MBP tag Monoclonal antibody","description":"Size: 150ul\nThe MBP tag (66003-1-Ig) by Proteintech is a Monoclonal antibody targeting MBP tag in WB, IF\/ICC, IP, ELISA applications with reactivity to recombinant protein samples\n66003-1-Ig targets MBP tag in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Recombinant protein, transfected HEK-293T cells\nPositive IP detected in: Recombinant protein protein\nPositive IF\/ICC detected in: Transfected cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nProtein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: recombinant protein\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag0942 Product name: Recombinant MBP-Tag protein Source: e coli. -derived, Unknow Tag: N\/A Domain: pMAL of Sequence: Predict reactive species\nFull Name: MBP tag\nCalculated Molecular Weight: 40 kDa\nObserved Molecular Weight: 40 kDa\nRRID: AB_11183040\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887978303657,"sku":"66003-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66003-1-ig","provider":"Iright","version":"1.0","type":"link"}