{"product_id":"proteintech-66071-1-ig","title":"Proteintech, 66071-1-Ig, EPLIN Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe EPLIN (66071-1-Ig) by Proteintech is a Monoclonal antibody targeting EPLIN in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, rat samples\n66071-1-Ig targets EPLIN in WB, IHC, IF\/ICC, CoIP, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, A431 cells,  COLO 320 cells,  HEK-293 cells,  human brain tissue,  HaCaT cells,  SCaBER cells,  MDA-MB-231 cells,  SK-BR-3 cells,  HT-29 cells,  pig colon tissue,  rat colon tissue,  HSC-T6 cells,  ROS1728 cells\nPositive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Hela cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag13288 Product name: Recombinant human LIMA1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 261-401 aa of BC001247 Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK Predict reactive species\nFull Name: LIM domain and actin binding 1\nCalculated Molecular Weight: 85 kDa\nObserved Molecular Weight: 90 kDa, 110 kDa\nGenBank Accession Number: BC001247\nGene Symbol: EPLIN\nGene ID (NCBI): 51474\nRRID: AB_11043341\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q9UHB6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888027619497,"sku":"66071-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66071-1-ig","provider":"Iright","version":"1.0","type":"link"}