{"product_id":"proteintech-66171-1-ig","title":"Proteintech, 66171-1-Ig, Transferrin\/TF Monoclonal antibody","description":"Size: 150ul\nThe Transferrin\/TF (66171-1-Ig) by Proteintech is a Monoclonal antibody targeting Transferrin\/TF in WB, IHC, IF-P, ELISA applications with reactivity to human samples\n66171-1-Ig targets Transferrin\/TF in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human plasma, fetal human brain tissue\nPositive IHC detected in: human liver cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human liver tissue, human placenta tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:200-1:2000\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nWhat is the tissue specificity of TF?Transferrin is synthesized mainly in the liver.What is the calculated molecular weight of TF?77 kDaHow many iron atoms can bind to TF?Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of ironand bicarbonatereleasesfree H+ ions, affinity decreases in acidic conditions, in which TFalso remains bound to its receptor.What is the primary role of TF?TF serves as a serum metal-binding protein, playing a key role in iron metabolism anddelivery for erythropoiesis.What is the role of TF in human diseases?TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia)in which there is adecrease in hepcidin expression, causing increased iron levels.Is TF essential for iron delivery?Congenital atransferrinemia results in an iron overload in some tissues, such as the liver,suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.In what circumstances do TF levels decrease?Inflammation and iron overload both can cause a decline in TF levels.How does TF affect hormonal balance?TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver.How does TF deficiency cause increased iron levels?TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption,resulting in TFdeficiency causing both anemia, and, paradoxically, increased iron levelsin some tissues.PMIDs: 23046645, 9635730, 22294463, 24589273\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag11668 Product name: Recombinant human TF protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 347-698 aa of BC059367 Sequence: EGTCQEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP Predict reactive species\nFull Name: transferrin\nCalculated Molecular Weight: 698 aa, 77 kDa\nObserved Molecular Weight: 77 kDa\nGenBank Accession Number: BC059367\nGene Symbol: TF\nGene ID (NCBI): 7018\nRRID: AB_2881566\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P02787\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888008614057,"sku":"66171-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66171-1-ig","provider":"Iright","version":"1.0","type":"link"}