{"product_id":"proteintech-66184-1-ig","title":"Proteintech, 66184-1-Ig, P62\/SQSTM1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe P62\/SQSTM1 (66184-1-Ig) by Proteintech is a Monoclonal antibody targeting P62\/SQSTM1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n66184-1-Ig targets P62\/SQSTM1 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, U2OS cells,  K-562 cells,  HEK-293 cells,  MCF-7 cells,  HepG2 cells,  Jurkat cells,  L02 cells,  Raji cells\nPositive IP detected in: U2OS cells\nPositive IHC detected in: human lung cancer tissue, human colon cancer tissue,  human liver cancer tissue,  human endometrial cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Chloroquine treated HepG2 cells, Starvation treated HepG2 cells,  U2OS cells,  Chloroquine treated U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSequestosome 1 (SQSTM1\/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras\/ Raf\/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies\/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48\/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, pig, canine, monkey, chicken, bovine, hamster\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag13131 Product name: Recombinant human P62;SQSTM1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-440 aa of BC017222 Sequence: MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL Predict reactive species\nFull Name: sequestosome 1\nCalculated Molecular Weight: 48 kDa\nObserved Molecular Weight: 62 kDa\nGenBank Accession Number: BC017222\nGene Symbol: P62\/SQSTM1\nGene ID (NCBI): 8878\nRRID: AB_2881579\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q13501\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887969783977,"sku":"66184-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66184-1-ig","provider":"Iright","version":"1.0","type":"link"}