{"product_id":"proteintech-66292-1-ig","title":"Proteintech, 66292-1-Ig, BDNF Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe BDNF (66292-1-Ig) by Proteintech is a Monoclonal antibody targeting BDNF in WB, IF\/ICC, ELISA applications with reactivity to human samples\n66292-1-Ig targets BDNF in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: U-251 cells, fetal human brain tissue\nPositive IF\/ICC detected in: U-251 cells, PC-12 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\n1) What is BDNF?BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.2) FAQs for BDNFA) I can detect more than one band in my samplesBDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, pig, zebrafish\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag11329 Product name: Recombinant human BDNF protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 21-247 aa of BC029795 Sequence: MKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHMIEELLDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR Predict reactive species\nFull Name: brain-derived neurotrophic factor\nCalculated Molecular Weight: 247 aa, 28 kDa\nObserved Molecular Weight: 32 kDa\nGenBank Accession Number: BC029795\nGene Symbol: BDNF\nGene ID (NCBI): 627\nRRID: AB_2881675\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P23560\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.\nFAQ\nA) I can detect more than one band in my samplesBDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).\nProtocolsProduct Specific ProtocolsIF protocol for BDNF antibody 66292-1-IgDownload protocolWB protocol for BDNF antibody 66292-1-IgDownload protocolStandard ProtocolsClick here to view our Standard Protocols\nProtocolsProduct Specific ProtocolsIF protocol for BDNF antibody 66292-1-IgDownload protocolWB protocol for BDNF antibody 66292-1-IgDownload protocolStandard ProtocolsClick here to view our Standard Protocols","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887979942057,"sku":"66292-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66292-1-ig","provider":"Iright","version":"1.0","type":"link"}