{"product_id":"proteintech-66375-1-ig","title":"Proteintech, 66375-1-Ig, TUBB3-specific\/TUJ1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe TUBB3-specific\/TUJ1 (66375-1-Ig) by Proteintech is a Monoclonal antibody targeting TUBB3-specific\/TUJ1 in WB, IHC, IF\/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat, pig, rabbit, chicken samples\n66375-1-Ig targets TUBB3-specific\/TUJ1 in WB, IHC, IF\/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SH-SY5Y cells, fetal human brain tissue,  HEK-293 cells,  PC-12 cells,  Neuro-2a cells,  Pig brain,  Rabbit brain,  Rat brain Mouse brain,  Chicken brain\nPositive IP detected in: SH-SY5Y cells\nPositive IHC detected in: human cerebellum tissue, mouse brain tissue,  mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: rat brain tissue, Retinal organoids,  mouse brain tissue,  human neuron\nPositive IF-Fro detected in: rat brain tissue, rat cerebellum tissue\nPositive IF\/ICC detected in: iPS cells\nPositive FC (Intra) detected in: SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:400-1:20000\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)-FRO: IF-FRO : 1:400-1:1600\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:125-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, rabbit, chicken\nCited Reactivity: human, mouse, rat, chicken\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tubulin, beta 3\nCalculated Molecular Weight: 55 kDa\nObserved Molecular Weight: 50-55 kDa\nGenBank Accession Number: NM_001197181\nGene Symbol: TUBB3\nGene ID (NCBI): 10381\nRRID: AB_2814998\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q13509\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887974994089,"sku":"66375-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66375-1-ig","provider":"Iright","version":"1.0","type":"link"}