{"product_id":"proteintech-66507-1-ig","title":"Proteintech, 66507-1-Ig, CNOT1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe CNOT1 (66507-1-Ig) by Proteintech is a Monoclonal antibody targeting CNOT1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples\n66507-1-Ig targets CNOT1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: U2OS cells, A549 cells,  HEK-293 cells,  human cerebellum tissue,  pig kidney tissue,  mouse kidney tissue,  HeLa cells,  LNCaP cells,  Jurkat cells,  K-562 cells,  HSC-T6 cells,  NIH\/3T3 cells\nPositive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag5623 Product name: Recombinant human CNOT1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-354 aa of BC040523 Sequence: MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNF Predict reactive species\nFull Name: CCR4-NOT transcription complex, subunit 1\nCalculated Molecular Weight: 267 kDa\nObserved Molecular Weight: 240-250 kDa\nGenBank Accession Number: BC040523\nGene Symbol: CNOT1\nGene ID (NCBI): 23019\nRRID: AB_2881870\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: A5YKK6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888026341545,"sku":"66507-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66507-1-ig","provider":"Iright","version":"1.0","type":"link"}