{"product_id":"proteintech-66625-1-ig","title":"Proteintech, 66625-1-Ig, RIC8A Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe RIC8A (66625-1-Ig) by Proteintech is a Monoclonal antibody targeting RIC8A in WB, IHC, IF-P, IP, ELISA applications with reactivity to Human, Rat, Mouse samples\n66625-1-Ig targets RIC8A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Rat, Mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, A431 cells,  HEK-293 cells,  Jurkat cells,  HSC-T6 cells,  RAW 264.7 cells,  NIH\/3T3 cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: mouse brain tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse brain tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, Rat, Mouse\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag1593 Product name: Recombinant human RIC8A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 117-467 aa of BC011821 Sequence: QQLFQELKGVRLLTDTLELTLGVTPEGNPPTLLPSQETERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD Predict reactive species\nFull Name: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)\nCalculated Molecular Weight: 60 kDa\nObserved Molecular Weight: 65 kDa\nGenBank Accession Number: BC011821\nGene Symbol: RIC8A\nGene ID (NCBI): 60626\nRRID: AB_2881985\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q9NPQ8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888049180841,"sku":"66625-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66625-1-ig","provider":"Iright","version":"1.0","type":"link"}