{"product_id":"proteintech-66807-1-ig","title":"Proteintech, 66807-1-Ig, PABPN1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe PABPN1 (66807-1-Ig) by Proteintech is a Monoclonal antibody targeting PABPN1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n66807-1-Ig targets PABPN1 in WB, IHC, IF\/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  HEK-293 cells,  MCF-7 cells,  Jurkat cells,  HSC-T6 cells,  NIH\/3T3 cells,  4T1 cells,  K-562 cells,  PC-12 cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human colon tissue, human colon cancer tissue,  rat heart tissue,  mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPoly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). \nAcetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag27470 Product name: Recombinant human PABPN1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 94-299 aa of BC010939 Sequence: GSQEEEEEPGLVEGDPGDGAIEDPELEAIKARVREMEEEAEKLKELQNEVEKQMNMSPPPGNAGPVIMSIEEKMEADARSIYVGNVDYGATAEELEAHFHGCGSVNRVTILCDKFSGHPKGFAYIEFSDKESVRTSLALDESLFRGRQIKVIPKRTNRPGISTTDRGFPRARYRARTTNYNSSRSRFYSGFNSRPRGRVYRGRARA Predict reactive species\nFull Name: poly(A) binding protein, nuclear 1\nCalculated Molecular Weight: 33 kDa\nObserved Molecular Weight: 50-56 kDa\nGenBank Accession Number: BC010939\nGene Symbol: PABPN1\nGene ID (NCBI): 8106\nRRID: AB_2882150\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q86U42\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888016216233,"sku":"66807-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66807-1-ig","provider":"Iright","version":"1.0","type":"link"}