{"product_id":"proteintech-66920-1-ig","title":"Proteintech, 66920-1-Ig, NFKB2 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe NFKB2 (66920-1-Ig) by Proteintech is a Monoclonal antibody targeting NFKB2 in WB, IHC, ELISA applications with reactivity to human samples\n66920-1-Ig targets NFKB2 in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, HAP1 cells,  WB result of NFKB2 antibody (66920-1-Ig; 1:1000; room temperature for 1.5 hours) with wild-type and NFKB2 knockout HAP1 cells.,  Jurkat cells,  K-562 cells,  Daudi cells,  THP-1 cells\nPositive IHC detected in: human liver cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:150-1:600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA\/p65, RELB, NFKB1\/p105, NFKB1\/p50, REL and NFKB2\/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag28543 Product name: Recombinant human NFKB2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 600-899 aa of BC002844 Sequence: GLYPVHLAVRARSPECLDLLVDSGAEVEATERQGGRTALHLATEMEELGLVTHLVTKLRANVNARTFAGNTPLHLAAGLGYPTLTRLLLKAGADIHAENEEPLCPLPSPPTSDSDSDSEGPEKDTRSSFRGHTPLDLTCSTKVKTLLLNAAQNTMEPPLTPPSPAGPGLSLGDTALQNLEQLLDGPEAQGSWAELAERLGLRSLVDTYRQTTSPSGSLLRSYELAGGDLAGLLEALSDMGLEEGVRLLRGPETRDKLPSTEVKEDSAYGSQSVEQEAEKLGPPPEPPGGLCHGHPQPQVH Predict reactive species\nFull Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49\/p100)\nCalculated Molecular Weight: 97 kDa\nObserved Molecular Weight: 100 kDa\nGenBank Accession Number: BC002844\nGene Symbol: NFKB2\nGene ID (NCBI): 4791\nENSEMBL Gene ID: ENSG00000077150\nRRID: AB_2882247\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q00653\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888305426601,"sku":"66920-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66920-1-ig","provider":"Iright","version":"1.0","type":"link"}