{"product_id":"proteintech-66934-1-ig","title":"Proteintech, 66934-1-Ig, PHEX Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe PHEX (66934-1-Ig) by Proteintech is a Monoclonal antibody targeting PHEX in WB, IHC, ELISA applications with reactivity to human, rat, pig samples\n66934-1-Ig targets PHEX in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, rat lung tissue,  pig lung tissue\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660). Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat, pig\nCited Reactivity: human, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag20290 Product name: Recombinant human PHEX protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 41-391 aa of BC105059 Sequence: SQGLLSLQAKQEYCLKPECIEAAAAILSKVNLSVDPCDNFFRFACDGWISNNPIPEDMPSYGVYPWLRHNVDLKLKELLEKSISRRRDTEAIQKAKILYSSCMNEKAIEKADAKPLLHILRHSPFRWPVLESNIGPEGVWSERKFSLLQTLATFRGQYSNSVFIRLYVSPDDKASNEHILKLDQATLSLAVREDYLDNSTEAKSYRDALYKFMVDTAVLLGANSSRAEHDMKSVLRLEIKIAEIMIPHENRTSEAMYNKMNISELSAMIPQFDWLGYIKKVIDTRLYPHLKDISPSENVVVRVPQYFKDLFRILGSERKKTIANYLVWRMVYSRIPNLSRRFQYRWLEFSR Predict reactive species\nFull Name: phosphate regulating endopeptidase homolog, X-linked\nCalculated Molecular Weight: 749 aa, 86 kDa\nObserved Molecular Weight: 76-80 kDa, 95-100 kDa\nGenBank Accession Number: BC105059\nGene Symbol: PHEX\nGene ID (NCBI): 5251\nRRID: AB_2882259\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P78562\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888047476905,"sku":"66934-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66934-1-ig","provider":"Iright","version":"1.0","type":"link"}