{"product_id":"proteintech-66999-1-ig","title":"Proteintech, 66999-1-Ig, NFIA Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe NFIA (66999-1-Ig) by Proteintech is a Monoclonal antibody targeting NFIA in WB, IP, ELISA applications with reactivity to Human, rat, mouse, pig, rabbit samples\n66999-1-Ig targets NFIA in WB, IP, ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, mouse cerebellum tissue,  fetal human brain tissue,  Jurkat cells,  pig brain tissue,  rat brain tissue,  mouse brain tissue,  rabbit brain tissue\nPositive IP detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found  in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, rat, mouse, pig, rabbit\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag17873 Product name: Recombinant human NFIA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-200 aa of BC022264 Sequence: MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDA Predict reactive species\nFull Name: nuclear factor I\/A\nCalculated Molecular Weight: 498 aa, 55 kDa\nObserved Molecular Weight: 60-70 kDa\nGenBank Accession Number: BC022264\nGene Symbol: NFIA\nGene ID (NCBI): 4774\nRRID: AB_2882316\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q12857\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888069038249,"sku":"66999-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-66999-1-ig","provider":"Iright","version":"1.0","type":"link"}