{"product_id":"proteintech-67337-1-ig","title":"Proteintech, 67337-1-Ig, SIRP Alpha\/CD172a Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe SIRP Alpha\/CD172a (67337-1-Ig) by Proteintech is a Monoclonal antibody targeting SIRP Alpha\/CD172a in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples\n67337-1-Ig targets SIRP Alpha\/CD172a in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: pig brain tissue, U-251 cells,  SW480 cells,  HL-60 cells,  THP-1 cells,  rat brain tissue,  mouse brain tissue,  rabbit brain tissue\nPositive IHC detected in: rat cerebellum tissue, human liver tissue,  rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a \"do not eat me\" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, rabbit\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag6361 Product name: Recombinant human SIRPA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 158-508 aa of BC033092 Sequence: ARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQVQSLDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK Predict reactive species\nFull Name: signal-regulatory protein alpha\nCalculated Molecular Weight: 55 kDa\nObserved Molecular Weight: 75-85 kDa\nGenBank Accession Number: BC033092\nGene Symbol: SIRP Alpha\nGene ID (NCBI): 140885\nRRID: AB_2882595\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P78324\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887966834857,"sku":"67337-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-67337-1-ig","provider":"Iright","version":"1.0","type":"link"}