{"product_id":"proteintech-67369-1-ig","title":"Proteintech, 67369-1-Ig, PNPLA3 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe PNPLA3 (67369-1-Ig) by Proteintech is a Monoclonal antibody targeting PNPLA3 in WB, IHC, IF\/ICC, ELISA applications with reactivity to Human, Rat, pig samples\n67369-1-Ig targets PNPLA3 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with Human, Rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  L02 cells,  SMMC-7721 cells,  HuH-7 cells,  HSC-T6 cells\nPositive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, Rat, pig\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag27407 Product name: Recombinant human PNPLA3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 229-339 aa of BC065195 Sequence: PDLKVLGEICLRGYLDAFRFLEEKGICNRPQPGLKSSSEGMDPEVAMPSWANMSLDSSPESAALAVRLEGDELLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYM Predict reactive species\nFull Name: patatin-like phospholipase domain containing 3\nObserved Molecular Weight: 53 kDa\nGenBank Accession Number: BC065195\nGene Symbol: PNPLA3\nGene ID (NCBI): 80339\nRRID: AB_2882619\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q9NST1\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888071397545,"sku":"67369-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-67369-1-ig","provider":"Iright","version":"1.0","type":"link"}