{"product_id":"proteintech-67500-1-ig","title":"Proteintech, 67500-1-Ig, RAN Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe RAN (67500-1-Ig) by Proteintech is a Monoclonal antibody targeting RAN in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples\n67500-1-Ig targets RAN in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  HepG2 cells,  Jurkat cells,  HSC-T6 cells,  NIH\/3T3 cells,  4T1 cells\nPositive IHC detected in: human colon cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human breast cancer tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: mouse\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag30034 Product name: Recombinant human RAN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-216 aa of BC004272 Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL Predict reactive species\nFull Name: RAN, member RAS oncogene family\nCalculated Molecular Weight: 25 kDa\nObserved Molecular Weight: 24 kDa\nGenBank Accession Number: BC004272\nGene Symbol: RAN\nGene ID (NCBI): 5901\nRRID: AB_2882724\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P62826\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888073298089,"sku":"67500-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-67500-1-ig","provider":"Iright","version":"1.0","type":"link"}