{"product_id":"proteintech-67512-1-ig","title":"Proteintech, 67512-1-Ig, BSEP Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe BSEP (67512-1-Ig) by Proteintech is a Monoclonal antibody targeting BSEP in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig samples\n67512-1-Ig targets BSEP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: LNCaP cells, unboiled HepG2 cells,  rat liver tissue,  mouse liver tissue,  pig lung tissue,  HeLa cells,  A549 cells\nPositive IHC detected in: mouse liver tissue, human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse liver tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:8000-1:32000\nImmunofluorescence (IF)-P: IF-P : 1:5000-1:20000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage. The calculated MW of ABCB11 is 146 kDa, 67512-1-Ig can detect bands around 150 kDa. It's reported that ABCB11 has several N-glycosylation sites, the observed higher molecular mass may due to posttranslational N-glycosylation. (PMID: 9545351, 24359682)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag29135 Product name: Recombinant human BSEP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 622-750 aa of NM_003742 Sequence: AADTIIGFEHGTAVERGTHEELLERKGVYFTLVTLQSQGNQALNEEDIKDATEDDMLARTFSRGSYQDSLRASIRQRSKSQLSYLVHEPPLAVVDHKSTYEEDRKDKDIPVQEEVEPAPVRRILKFSAP Predict reactive species\nFull Name: ATP-binding cassette, sub-family B (MDR\/TAP), member 11\nCalculated Molecular Weight: 146 kDa\nObserved Molecular Weight: 150-160 kDa\nGenBank Accession Number: NM_003742\nGene Symbol: BSEP\nGene ID (NCBI): 8647\nRRID: AB_2882733\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: O95342\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887995900073,"sku":"67512-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-67512-1-ig","provider":"Iright","version":"1.0","type":"link"}