{"product_id":"proteintech-67661-1-ig","title":"Proteintech, 67661-1-Ig, ETFA Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe ETFA (67661-1-Ig) by Proteintech is a Monoclonal antibody targeting ETFA in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples\n67661-1-Ig targets ETFA in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HCT 116 cells, LNCaP cells,  mouse brain tissue,  HuH-7 cells,  HeLa cells,  K-562 cells,  pig liver tissue,  rat liver tissue\nPositive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:8000-1:32000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag30257 Product name: Recombinant human ETFA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-333 aa of BC015526 Sequence: MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK Predict reactive species\nFull Name: electron-transfer-flavoprotein, alpha polypeptide\nCalculated Molecular Weight: 30 kDa\nObserved Molecular Weight: 30 kDa\nGenBank Accession Number: BC015526\nGene Symbol: ETFA\nGene ID (NCBI): 2108\nRRID: AB_2882858\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P13804\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888061960361,"sku":"67661-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-67661-1-ig","provider":"Iright","version":"1.0","type":"link"}