{"product_id":"proteintech-67676-1-ig","title":"Proteintech, 67676-1-Ig, PCK2 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe PCK2 (67676-1-Ig) by Proteintech is a Monoclonal antibody targeting PCK2 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples\n67676-1-Ig targets PCK2 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HuH-7 cells, rat liver tissue,  pig liver tissue,  rabbit liver tissue,  HepG2 cells,  LNCaP cells,  K562 cells,  Neuro-2a cells,  HSC-T6 cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human lung cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:4000-1:16000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, rabbit\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag7021 Product name: Recombinant human PCK2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 292-640 aa of BC001454 Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM Predict reactive species\nFull Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)\nCalculated Molecular Weight: 71 kDa\nObserved Molecular Weight: 68 kDa\nGenBank Accession Number: BC001454\nGene Symbol: PCK2\nGene ID (NCBI): 5106\nRRID: AB_2882871\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q16822\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888022442153,"sku":"67676-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-67676-1-ig","provider":"Iright","version":"1.0","type":"link"}