{"product_id":"proteintech-67735-1-ig","title":"Proteintech, 67735-1-Ig, Alpha smooth muscle actin specific Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Alpha smooth muscle actin specific (67735-1-Ig) by Proteintech is a Monoclonal antibody targeting Alpha smooth muscle actin specific in WB, IHC, IF\/ICC, IF-P, IF-Fro, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples\n67735-1-Ig targets Alpha smooth muscle actin specific in WB, IHC, IF\/ICC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rat stomach tissue, rabbit stomach tissue,  pig vein tissue,  pig stomach tissue,  rabbit large intestine tissue,  pig colon tissue,  rat colon tissue,  mouse colon tissue\nPositive IHC detected in: human colon tissue, human appendicitis tissue,  human liver tissue,  human liver cancer tissue,  rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: rat heart tissue\nPositive IF-Fro detected in: mouse breast cancer\nPositive IF\/ICC detected in: C2C12 cells\nPositive FC (Intra) detected in: C2C12 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:20000-1:100000\nImmunohistochemistry (IHC): IHC : 1:2500-1:10000\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\nImmunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, rabbit\nCited Reactivity: human, mouse, rat, pig, zebrafish\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: actin, alpha 2, smooth muscle, aorta\nCalculated Molecular Weight: 42 kDa\nObserved Molecular Weight: 42 kDa\nGenBank Accession Number: NM_001613\nGene Symbol: Alpha smooth muscle actin\nGene ID (NCBI): 59\nRRID: AB_2918504\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P62736\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887969325225,"sku":"67735-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-67735-1-ig","provider":"Iright","version":"1.0","type":"link"}