{"product_id":"proteintech-67989-1-ig","title":"Proteintech, 67989-1-Ig, MCM6 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe MCM6 (67989-1-Ig) by Proteintech is a Monoclonal antibody targeting MCM6 in WB, IHC, IF\/ICC, ELISA applications with reactivity to Human, Rat, Mouse samples\n67989-1-Ig targets MCM6 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, HeLa cells,  HEK-293 cells,  COLO 320 cells,  HepG2 cells,  MCF-7 cells,  Jurkat cells,  PC-12 cells,  NIH\/3T3 cells\nPositive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:20000-1:100000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:2000-1:8000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit  forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, Rat, Mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag4354 Product name: Recombinant human MCM6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 471-821 aa of BC032374 Sequence: VAIHEAMEQQTISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED Predict reactive species\nFull Name: minichromosome maintenance complex component 6\nCalculated Molecular Weight: 821 aa, 93 kDa\nObserved Molecular Weight: 105 kDa\nGenBank Accession Number: BC032374\nGene Symbol: MCM6\nGene ID (NCBI): 4175\nRRID: AB_2918738\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q14566\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888291598505,"sku":"67989-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-67989-1-ig","provider":"Iright","version":"1.0","type":"link"}