{"product_id":"proteintech-68015-1-ig","title":"Proteintech, 68015-1-Ig, Phospho-MEK1 (Thr386) Monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-MEK1 (Thr386) (68015-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-MEK1 (Thr386) in WB, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n68015-1-Ig targets Phospho-MEK1 (Thr386) in WB, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  A431 cells,  NIH\/3T3 cells,  nocodazole treated HEK-293 cells,  nocodazole treated A431 cells,  Calyculin A treated NIH\/3T3 cells,  Calyculin A treated HeLa cells,  λ phosphatase treated HeLa cells\nPositive IF\/ICC detected in: Calyculin A treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK\/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: mitogen-activated protein kinase kinase 1\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 40-50 kDa\nGenBank Accession Number: BC139729\nGene Symbol: MEK1\nGene ID (NCBI): 5604\nENSEMBL Gene ID: ENSG00000169032\nRRID: AB_2918761\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q02750\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888070906025,"sku":"68015-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-68015-1-ig","provider":"Iright","version":"1.0","type":"link"}