{"product_id":"proteintech-68151-1-ig","title":"Proteintech, 68151-1-Ig, DOHH Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe DOHH (68151-1-Ig) by Proteintech is a Monoclonal antibody targeting DOHH in WB, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n68151-1-Ig targets DOHH in WB, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: LNCaP cells, HSC-T6 cells,  NIH\/3T3 cells,  HeLa cells,  HEK-293 cells,  Jurkat cells\nPositive IF\/ICC detected in: LNCaP cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDeoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH\/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag13041 Product name: Recombinant human DOHH protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-302 aa of BC002817 Sequence: MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS Predict reactive species\nFull Name: deoxyhypusine hydroxylase\/monooxygenase\nCalculated Molecular Weight: 33 kDa\nObserved Molecular Weight: 30-35 kDa\nGenBank Accession Number: BC002817\nGene Symbol: DOHH\nGene ID (NCBI): 83475\nRRID: AB_2935259\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q9BU89\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888272953513,"sku":"68151-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-68151-1-ig","provider":"Iright","version":"1.0","type":"link"}