{"product_id":"proteintech-68169-1-ig","title":"Proteintech, 68169-1-Ig, NIT2 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe NIT2 (68169-1-Ig) by Proteintech is a Monoclonal antibody targeting NIT2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to Human, mouse, rat, pig samples\n68169-1-Ig targets NIT2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  pig liver tissue,  mouse liver tissue,  HepG2 cells,  Jurkat cells\nPositive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U-251 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, mouse, rat, pig\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG2b\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag9889 Product name: Recombinant human NIT2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-276 aa of BC020620 Sequence: MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIPGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEALAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMKKP Predict reactive species\nFull Name: nitrilase family, member 2\nCalculated Molecular Weight: 276 aa, 31 kDa\nObserved Molecular Weight: 31 kDa\nGenBank Accession Number: BC020620\nGene Symbol: NIT2\nGene ID (NCBI): 56954\nRRID: AB_2923685\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9NQR4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888069234857,"sku":"68169-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-68169-1-ig","provider":"Iright","version":"1.0","type":"link"}