{"product_id":"proteintech-68251-1-ig","title":"Proteintech, 68251-1-Ig, TPMT Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe TPMT (68251-1-Ig) by Proteintech is a Monoclonal antibody targeting TPMT in WB, IF\/ICC, ELISA applications with reactivity to Human samples\n68251-1-Ig targets TPMT in WB, IF\/ICC, ELISA applications and shows reactivity with Human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  HepG2 cells,  Jurkat cells,  k-562 cells\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag28693 Product name: Recombinant human TPMT protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-245 aa of BC005339 Sequence: MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRRLEKVDAFEERHKSWGIDCLFEKLYLLTEK Predict reactive species\nFull Name: thiopurine S-methyltransferase\nCalculated Molecular Weight: 28 kDa\nObserved Molecular Weight: 32 kDa\nGenBank Accession Number: BC005339\nGene Symbol: TPMT\nGene ID (NCBI): 7172\nRRID: AB_2935336\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P51580\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888332296361,"sku":"68251-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-68251-1-ig","provider":"Iright","version":"1.0","type":"link"}