{"product_id":"proteintech-68297-1-ig","title":"Proteintech, 68297-1-Ig, MICAL1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe MICAL1 (68297-1-Ig) by Proteintech is a Monoclonal antibody targeting MICAL1 in WB, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n68297-1-Ig targets MICAL1 in WB, IF\/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, RAW 264.7 cells,  HSC-T6 cells,  HeLa cells,  HEK-293 cells,  HepG2 cells,  Jurkat cells,  MOLT-4 cells,  K-562 cells\nPositive IP detected in: HeLa cells\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:1000-1:4000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag6578 Product name: Recombinant human MICAL1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 718-1067 aa of BC052983 Sequence: HRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESPELPTPSENSMPPGLSTPTASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNLTPDPEMEPPPKPPRSCSALARHALESSFVGWGLPVQSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQALQTFAKTSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQTIQRRLNEIEAALRELEAEGVKLELALRRQSSSPEQQKKLWVGQLLQLVDKKNSLVAEEAELMITVQELNLEEKQWQLDQELRGYMNREENLKTAADRQAEDQVLRKLVDLVNQRDALIRFQEERRLSELALGTGAQG Predict reactive species\nFull Name: microtubule associated monoxygenase, calponin and LIM domain containing 1\nCalculated Molecular Weight: 118 kDa\nObserved Molecular Weight: 120 kDa\nGenBank Accession Number: BC052983\nGene Symbol: MICAL1\nGene ID (NCBI): 64780\nRRID: AB_2935377\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q8TDZ2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888293073065,"sku":"68297-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-68297-1-ig","provider":"Iright","version":"1.0","type":"link"}