{"product_id":"proteintech-68393-1-ig","title":"Proteintech, 68393-1-Ig, Histone H2B Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe Histone H2B (68393-1-Ig) by Proteintech is a Monoclonal antibody targeting Histone H2B in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat, chicken, zebrafish samples\n68393-1-Ig targets Histone H2B in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, chicken, zebrafish samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: LNCaP cells, HeLa cells,  HEK-293 cells,  Jurkat cells,  HSC-T6 cells,  NIH\/3T3 cells,  chicken cerebellum tissue,  zebrafish tissue\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHistones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome\/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, chicken, zebrafish\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: histone cluster 2, H2be\nCalculated Molecular Weight: 14 kDa\nObserved Molecular Weight: 14-17 kDa\nGenBank Accession Number: BC005827\nGene Symbol: Histone H2B\nGene ID (NCBI): 8349\nRRID: AB_3085110\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q16778\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888064450729,"sku":"68393-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-68393-1-ig","provider":"Iright","version":"1.0","type":"link"}