{"product_id":"proteintech-68579-1-ig","title":"Proteintech, 68579-1-Ig, 5-Hydroxymethylcytidine Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe 5-Hydroxymethylcytidine (68579-1-Ig) by Proteintech is a Monoclonal antibody targeting 5-Hydroxymethylcytidine in Dot Blot, ELISA applications with reactivity to chemical compound samples\n68579-1-Ig targets 5-Hydroxymethylcytidine in Dot Blot, ELISA applications and shows reactivity with chemical compound samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive ELISA detected in: 5-Hydroxymethylcytidine\nPositive Dot Blot detected in: RNA\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nEnzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:2000-1:20000\nDOT BLOT: DOT BLOT : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nOxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. Recent reaearch shows that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA. It is known that RNA carries more than 100 distinct types of modifications, and these modifications modulate the structure and functions of RNA. Ribonucleoside 5-methylcytidine (m5C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm5C) and 5-formylcytidine (f5C). Researchers have identified hm5C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m5C oxidation is a conserved process that could have critical regulatory functions inside cells (PMID: 25676849).Protocol for Dot Blot:https:\/\/www.ptglab.com\/protocol\/68579-1-IgDotBlot.pdf\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: chemical compound\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: PTG Predict reactive species\nFull Name: 5-Hydroxymethylcytidine\nRRID: AB_3085279\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888080867497,"sku":"68579-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-68579-1-ig","provider":"Iright","version":"1.0","type":"link"}