{"product_id":"proteintech-80008-1-rr","title":"Proteintech, 80008-1-RR, Alpha smooth muscle actin specific Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Alpha smooth muscle actin specific (80008-1-RR) by Proteintech is a Recombinant antibody targeting Alpha smooth muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig, chicken, zebrafish samples\n80008-1-RR targets Alpha smooth muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse colon tissue, pig vein tissue,  chicken crop tissue,  zebrafish tissue,  A549 cells,  pig stomach,  pig colon,  rat stomach,  chicken small intestine tissue,  rat colon tissue\nPositive IHC detected in: human liver tissue, human colon tissue,  mouse colon tissue,  rat colon tissue,  human heart tissue,  human appendicitis tissue,  human rectal cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse heart tissue, mouse embryo tissue\nPositive IF-Fro detected in: mouse small intestine tissue\nPositive FC (Intra) detected in: C2C12 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:20000-1:100000\nImmunohistochemistry (IHC): IHC : 1:2500-1:10000\nImmunofluorescence (IF)-P: IF-P : 1:500-1:2000\nImmunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig, chicken, zebrafish\nCited Reactivity: human, mouse, rat, pig, duck\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: actin, alpha 2, smooth muscle, aorta\nCalculated Molecular Weight: 42 kDa\nObserved Molecular Weight: 42 kDa\nGenBank Accession Number: NM_001613\nGene Symbol: Alpha smooth muscle actin\nGene ID (NCBI): 59\nRRID: AB_2882938\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P62736\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888341209257,"sku":"80008-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-80008-1-rr","provider":"Iright","version":"1.0","type":"link"}