{"product_id":"proteintech-80024-1-rr","title":"Proteintech, 80024-1-RR, Phospho-JNK (Tyr185) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-JNK (Tyr185) (80024-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-JNK (Tyr185) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples\n80024-1-RR targets Phospho-JNK (Tyr185) in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, Calyculin A treated HEK-293T cells,  UV treated HEK-293T cells,  UV treated NIH\/3T3 cells,  Anisomycin treated NIH\/3T3 cells\nPositive IF\/ICC detected in: UV treated HEK-293 cells\nPositive FC (Intra) detected in: Calyculin A treated HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2\/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, pig, zebrafish, sheep\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: mitogen-activated protein kinase 8\nObserved Molecular Weight: 37-46 kDa\nGenBank Accession Number: NM_139049\nGene Symbol: JNK\nGene ID (NCBI): 5599\nRRID: AB_2882943\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P45983\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888340521129,"sku":"80024-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-80024-1-rr","provider":"Iright","version":"1.0","type":"link"}