{"product_id":"proteintech-80093-1-rr","title":"Proteintech, 80093-1-RR, Phospho-MST1 (Thr183)\/MST2 (Thr180) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-MST1 (Thr183)\/MST2 (Thr180) (80093-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-MST1 (Thr183)\/MST2 (Thr180) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples\n80093-1-RR targets Phospho-MST1 (Thr183)\/MST2 (Thr180) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, HeLa cells,  Calyculin A treated NIH\/3T3 cells,  Calyculin A treated HeLa cells,  Staurosporine treated Ramos cells,  Staurosporine treated Jurkat cells\nPositive FC (Intra) detected in: Calyculin A treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3\/4 and LATS1\/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3\/4 and LATS1\/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors.\nLines of evidence have indicated that dysregulation or loss of STK4\/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4\/3 because phosphorylation of Thr183\/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: serine\/threonine kinase 4\nCalculated Molecular Weight: 56 kDa\nObserved Molecular Weight: 59 kDa\nGenBank Accession Number: BC005231\nGene Symbol: MST1\nGene ID (NCBI): 6789\nRRID: AB_2918864\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q13043\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888362573993,"sku":"80093-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-80093-1-rr","provider":"Iright","version":"1.0","type":"link"}