{"product_id":"proteintech-80103-2-rr","title":"Proteintech, 80103-2-RR, Phospho-GRB10 (Ser476) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-GRB10 (Ser476) (80103-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-GRB10 (Ser476) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples\n80103-2-RR targets Phospho-GRB10 (Ser476) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: NIH\/3T3 cells, λ phosphatase treated NIH\/3T3 cells\nPositive FC (Intra) detected in: λ phosphatase treated HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGrowth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2\/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic\/mammalian target of mTOR and can be  phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: growth factor receptor-bound protein 10\nCalculated Molecular Weight: 536aa,61 kDa; 594aa,67 kDa\nObserved Molecular Weight: 70-80 kDa\nGenBank Accession Number: BC024285\nGene Symbol: GRB10\nGene ID (NCBI): 2887\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q13322\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888801501353,"sku":"80103-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-80103-2-rr","provider":"Iright","version":"1.0","type":"link"}