{"product_id":"proteintech-80130-2-rr","title":"Proteintech, 80130-2-RR, Phospho-S6 Ribosomal protein (Ser235\/236) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-S6 Ribosomal protein (Ser235\/236) (80130-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-S6 Ribosomal protein (Ser235\/236) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n80130-2-RR targets Phospho-S6 Ribosomal protein (Ser235\/236) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: PDGF treated HEK-293 cells, PDGF treated C6 cells,  PDGF treated NIH\/3T3 cells\nPositive IF\/ICC detected in: 100 nM Calyculin A (30 minutes) treated HeLa cells\nPositive FC (Intra) detected in: 100 nM Calyculin A (30 minutes) treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235\/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ribosomal protein S6\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: NM_001010\nGene Symbol: RPS6\nGene ID (NCBI): 6194\nRRID: AB_3670467\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P62753\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888534900905,"sku":"80130-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-80130-2-rr","provider":"Iright","version":"1.0","type":"link"}