{"product_id":"proteintech-80340-1-rr","title":"Proteintech, 80340-1-RR, Phospho-Hamartin\/TSC1 (Ser511) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-Hamartin\/TSC1 (Ser511) (80340-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Hamartin\/TSC1 (Ser511) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples\n80340-1-RR targets Phospho-Hamartin\/TSC1 (Ser511) in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: NIH\/3T3 cells, HeLa cells,  Calyculin A treated HeLa cells,  Calyculin A treated HEK-293 cells,  Calyculin A treated NIH\/3T3 cells\nPositive FC (Intra) detected in: Calyculin A treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)\/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1\/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1\/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1  is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tuberous sclerosis 1\nCalculated Molecular Weight: 130 kDa\nObserved Molecular Weight: 150 kDa\nGenBank Accession Number: NM_000368\nGene Symbol: Hamartin\/TSC1\nGene ID (NCBI): 7248\nRRID: AB_2918889\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q92574\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888377352361,"sku":"80340-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-80340-1-rr","provider":"Iright","version":"1.0","type":"link"}