{"product_id":"proteintech-80427-2-rr","title":"Proteintech, 80427-2-RR, Phospho-SMAD2 (Ser465\/467) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-SMAD2 (Ser465\/467) (80427-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-SMAD2 (Ser465\/467) in WB, IF\/ICC, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples\n80427-2-RR targets Phospho-SMAD2 (Ser465\/467) in WB, IF\/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated HEK-293 cells, Calyculin A treated HeLa cells\nPositive IF\/ICC detected in: TGF beta 1 treated HEK-293 cells\nPositive FC (Intra) detected in: Calyculin A treated HEK-293 cells\nPositive ChIP-qPCR detected in: TGF-β1 (7 ng\/ml, 1 h) HaCaT cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSMAD2, also named as MADH2 and MADR2, belongs to the dwarfin\/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain.  SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases.  This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465\/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity.  The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)The antibody recognizes both SMAD3 protein phosphorylated at Ser423\/425.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: SMAD family member 2\nCalculated Molecular Weight: 467 aa, 52 kDa\nObserved Molecular Weight: 65 kDa\nGenBank Accession Number: BC014840\nGene Symbol: SMAD2\nGene ID (NCBI): 4087\nRRID: AB_3670477\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q15796\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888683176105,"sku":"80427-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-80427-2-rr","provider":"Iright","version":"1.0","type":"link"}