{"product_id":"proteintech-80431-2-rr","title":"Proteintech, 80431-2-RR, Phospho-BAD (Ser112) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-BAD (Ser112) (80431-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-BAD (Ser112) in WB, FC (Intra), ELISA applications with reactivity to human samples\n80431-2-RR targets Phospho-BAD (Ser112) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells\nPositive FC (Intra) detected in: λ phosphatase treated HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBAD, a distant member of the BCL-2 family, exerts its death promoting e€ect by hetero-dimerizing with BCL-XL and BCL-2 death antagonists in the mitochondria. The function of the pro-apoptotic molecule BAD is regulated by phosphorylation of two sites, serine-112 (Ser-112) and serine-136 (Ser-136). Phosphorylation at either site results in loss of the ability of BAD to heterodimerize with the survival proteins BCL-XL or BCL-2. Phosphorylated BAD binds to 14-3-3 and is sequestered in the cytoplasm. (PMID: 10597268)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: BCL2-associated agonist of cell death\nCalculated Molecular Weight: 18 kDa\nObserved Molecular Weight: 25 kDa\nGenBank Accession Number: BC001901\nGene Symbol: BAD\nGene ID (NCBI): 572\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q92934\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888799797417,"sku":"80431-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-80431-2-rr","provider":"Iright","version":"1.0","type":"link"}