{"product_id":"proteintech-81004-1-rr","title":"Proteintech, 81004-1-RR, LC3B Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe LC3B (81004-1-RR) by Proteintech is a Recombinant antibody targeting LC3B in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples\n81004-1-RR targets LC3B in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, HEK-293 cells,  HeLa cells,  Chloroquine treated HEK-293 cells,  Chloroquine treated HeLa cells\nPositive IHC detected in: human colon cancer tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Chloroquine treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMap1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. The antibody 81004-1-RR specifically recognizes LC3B.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, pig\nCited Reactivity: human, mouse, rat, bovine, sheep\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag6144 Product name: Recombinant human LC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC067797 Sequence: MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMGELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV Predict reactive species\nFull Name: microtubule-associated protein 1 light chain 3 beta\nCalculated Molecular Weight: 15 kDa\nObserved Molecular Weight: 14-18 kDa\nGenBank Accession Number: BC067797\nGene Symbol: LC3B\nGene ID (NCBI): 81631\nENSEMBL Gene ID: ENSG00000140941\nRRID: AB_2923695\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9GZQ8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888341405865,"sku":"81004-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-81004-1-rr","provider":"Iright","version":"1.0","type":"link"}