{"product_id":"proteintech-81475-1-rr","title":"Proteintech, 81475-1-RR, Phospho-RPS6KA1 (Thr359\/Ser363) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-RPS6KA1 (Thr359\/Ser363) (81475-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-RPS6KA1 (Thr359\/Ser363) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples\n81475-1-RR targets Phospho-RPS6KA1 (Thr359\/Ser363) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, λ phosphatase treated HeLa cells\nPositive FC (Intra) detected in: λ phosphatase treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine\/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1\/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1\/ER81 and NR4A1\/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1\nCalculated Molecular Weight: 735 aa, 83 kDa\nObserved Molecular Weight: 83-90 kDa\nGenBank Accession Number: BC014966\nGene Symbol: RPS6KA1\nGene ID (NCBI): 6195\nRRID: AB_2935552\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q15418\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888656371881,"sku":"81475-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-81475-1-rr","provider":"Iright","version":"1.0","type":"link"}