{"product_id":"proteintech-81691-2-rr","title":"Proteintech, 81691-2-RR, CHMP4B Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe CHMP4B (81691-2-RR) by Proteintech is a Recombinant antibody targeting CHMP4B in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples\n81691-2-RR targets CHMP4B in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, HeLa cells,  HuH-7 cells,  C2C12 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag4544 Product name: Recombinant human CHMP4B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC033859 Sequence: MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM Predict reactive species\nFull Name: chromatin modifying protein 4B\nCalculated Molecular Weight: 224 aa, 25 kDa\nObserved Molecular Weight: 25 kDa\nGenBank Accession Number: BC033859\nGene Symbol: CHMP4B\nGene ID (NCBI): 128866\nRRID: AB_3670502\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q9H444\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888404418729,"sku":"81691-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-81691-2-rr","provider":"Iright","version":"1.0","type":"link"}