{"product_id":"proteintech-81871-1-rr","title":"Proteintech, 81871-1-RR, Phospho-CREB1 (Ser133) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-CREB1 (Ser133) (81871-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-CREB1 (Ser133) in WB, IHC, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples\n81871-1-RR targets Phospho-CREB1 (Ser133) in WB, IHC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated HEK-293 cells\nPositive IHC detected in: human prostate cancer tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive ChIP-qPCR detected in: Forskolin (30 μM, 1 h) HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB\/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: cAMP responsive element binding protein 1\nCalculated Molecular Weight: 341 aa, 35 kDa\nObserved Molecular Weight: 35-46 kDa\nGenBank Accession Number: BC010636\nGene Symbol: CREB1\nGene ID (NCBI): 1385\nRRID: AB_2935430\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P16220\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888682225833,"sku":"81871-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-81871-1-rr","provider":"Iright","version":"1.0","type":"link"}