{"product_id":"proteintech-82309-1-rr","title":"Proteintech, 82309-1-RR, Phospho-RASGRF1 (Ser927) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-RASGRF1 (Ser927) (82309-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-RASGRF1 (Ser927) in WB, ELISA applications with reactivity to human samples\n82309-1-RR targets Phospho-RASGRF1 (Ser927) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Forskolin treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:5000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: Ras protein-specific guanine nucleotide-releasing factor 1\nCalculated Molecular Weight: 134 kDa\nObserved Molecular Weight: 145 kDa\nGenBank Accession Number: BC040275\nGene Symbol: RASGRF1\nGene ID (NCBI): 5923\nRRID: AB_3085358\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q13972\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888534769833,"sku":"82309-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-82309-1-rr","provider":"Iright","version":"1.0","type":"link"}