{"product_id":"proteintech-82619-4-rr","title":"Proteintech, 82619-4-RR, Glucocorticoid receptor Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Glucocorticoid receptor (82619-4-RR) by Proteintech is a Recombinant antibody targeting Glucocorticoid receptor in WB, IHC, IF\/ICC, IP, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples\n82619-4-RR targets Glucocorticoid receptor in WB, IHC, IF\/ICC, IP, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, A549 cells,  U-251 cells,  DU 145 cells,  MCF-7 cells,  L-929 cells,  RAW 264.7 cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human prostate cancer tissue, human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\nPositive ChIP-qPCR detected in: dexamethasone (100 nM, 1 h) A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:175-1:700\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGlucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag21146 Product name: Recombinant human Glucocorticoid receptor protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC015610 Sequence: MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPI Predict reactive species\nFull Name: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)\nCalculated Molecular Weight: 86 kDa\nObserved Molecular Weight: 94-97 kDa\nGenBank Accession Number: BC015610\nGene Symbol: Glucocorticoid receptor\nGene ID (NCBI): 2908\nRRID: AB_3086492\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P04150\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888355659945,"sku":"82619-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-82619-4-rr","provider":"Iright","version":"1.0","type":"link"}