{"product_id":"proteintech-82717-1-rr","title":"Proteintech, 82717-1-RR, Phospho-Myosin Light Chain 2 (Thr18\/Ser19) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-Myosin Light Chain 2 (Thr18\/Ser19) (82717-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Myosin Light Chain 2 (Thr18\/Ser19) in WB, ELISA applications with reactivity to human, mouse samples\n82717-1-RR targets Phospho-Myosin Light Chain 2 (Thr18\/Ser19) in WB, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated NIH\/3T3 cells, Calyculin A treated HeLa cells\nPositive FC (Intra) detected in: Calyculin A treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMyosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs\/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18\/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: myosin, light chain 9, regulatory\nCalculated Molecular Weight: 20 kDa\nObserved Molecular Weight: 19-20 kDa\nGenBank Accession Number: BC002648\nGene Symbol: MYL9\nGene ID (NCBI): 10398\nRRID: AB_3085363\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P24844\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888531132585,"sku":"82717-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-82717-1-rr","provider":"Iright","version":"1.0","type":"link"}