{"product_id":"proteintech-82736-11-rr","title":"Proteintech, 82736-11-RR, Phospho-EGFR (Tyr1016) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-EGFR (Tyr1016) (82736-11-RR) by Proteintech is a Recombinant antibody targeting Phospho-EGFR (Tyr1016) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n82736-11-RR targets Phospho-EGFR (Tyr1016) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: EGF treated A431 cells\nPositive IF\/ICC detected in: EGF treated A431 cells\nPositive FC (Intra) detected in: EGF treated A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEpidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB\/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Phosphorylation at Tyr1016 is essential for the recruitment of downstream signaling molecules. For example, it can interact with adapter proteins like GRB2, which in turn activates the Ras-MAPK pathway, leading to cell proliferation and differentiation. (PMID: 26628682) Some scientists call EGFR Tyr1016 as EGFR Tyr992, the site where the signaling peptide is removed.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)\nCalculated Molecular Weight: 1210 aa, 134 kDa\nObserved Molecular Weight: 170 kDa\nGenBank Accession Number: BC094761\nGene Symbol: EGFR\nGene ID (NCBI): 1956\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P00533\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888800878761,"sku":"82736-11-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-82736-11-rr","provider":"Iright","version":"1.0","type":"link"}