{"product_id":"proteintech-82751-5-rr","title":"Proteintech, 82751-5-RR, Phospho-CDK1 (Tyr15) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-CDK1 (Tyr15) (82751-5-RR) by Proteintech is a Recombinant antibody targeting Phospho-CDK1 (Tyr15) in WB, FC (Intra), ELISA applications with reactivity to human samples\n82751-5-RR targets Phospho-CDK1 (Tyr15) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, λ phosphatase treated HeLa cells\nPositive FC (Intra) detected in: λ phosphatase treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser\/Thr protein kinase family and CDC2\/CDKX subfamily. Phospho-CDK1 (Tyr15) is the inactive, inhibited form of cyclin-dependent kinase 1 (CDK1) generated when the tyrosine 15 residue of CDK1's ATP-binding loop is phosphorylated by the kinases WEE1 and PKMYT1.  This single phosphorylation event clamps the kinase in an inactive conformation, preventing premature onset of mitosis by blocking ATP binding and cyclin B1 activation. (PMID: 14645578)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: cell division cycle 2, G1 to S and G2 to M\nCalculated Molecular Weight: 25 kDa\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: BC014563\nGene Symbol: CDK1\nGene ID (NCBI): 983\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P06493\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888800157865,"sku":"82751-5-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-82751-5-rr","provider":"Iright","version":"1.0","type":"link"}